Investigation of Proapoptotic Potential of Ipomoea carnea Leaf Extract on Breast Cancer Cell Line

Cancer remains to be a major global healthcare burden as 18.1 million new cases and 96 death occurred 2018 on the basis of report from international agency for research cancer Breast is most common diagnosed in women causing highest morbidity. Ipomoea carnea leaf extracts have been used chemotherapeutic agent due their antioxidant, anti-inflammatory, anti-bacterial, anti-tumor effect. The aim study evaluate proapoptotic potential extract breast cell lines (MCF-7). was assessed using an MTT assay. morphological changes extract-treated cells were observed under phase-contrast microscope. effect examined DAPI staining. assay results showed significant reduction viability after treatment with different concentrations (5-80 µg/ml) 24h. We inhibitory concentration at 20 ?g/ml. Morphological such number cells, shrinkage, cytoplasmic membrane blebbing indicate hallmark features apoptosis cells. staining that apoptotic nuclei are stained intensely, fragmented, condensation chromatin treated Therefore it can concluded pro-apoptotic (MCF-7).
 Keywords: carnea, cancer, MCF-7, Apoptosis